YEAST AND MOLD

Yeast and Mold

Molds is a type of fungus that is micellular and grow filamentous branches called hyphae. Each cell has identical nuclei and they grow in colonies as a single organism. Yeast are single celled microbes are white and thread like whilst molds can be green, orange, black, brown, pink or purple.

Occurrence of Yeast and Molds

Molds tend to grow in damp dark areas whilst yeast can be found everywhere on skin, in stomachs of animals, on berries and plants. Yeast and molds are highly adaptive of their environments and can grow over a wide range of temperatures and pH values. There are about 1000 species of molds and about 1500 species of yeast. Molds reproduce asexually or sexually through spores whilst yeast grows by mitosis or budding. Yeast and molds are responsible for the spoilage of food products. Some molds develop mycotoxins which can cause acute or chronic poisoning. Due to their widespread prevalence the enumeration of yeast and molds is an important parameter for HACCP.

Conventional Method - Biolife Italiana

Arasains Indonesia supplies potato dextrose agar from Biolife for the enumeration of yeast and molds. The PDA must be acidified with 2ml of sterile 10% tartaric acid prior to pouring onto petri dishes. Mycological agar and YM 11 Agar with chlorotetracycline are also available.

Film Method - MC Media Pad YM

Merck Indonesia supplies the MC-Media Pad® that designed for convenient and rapid routine testing of microbial contaminations in your food and beverage products. Convenient culture media for enumeration of total yeast and mold count. The MC-Media Pad® is ready-to-use and provides additional features for your cost-efficiency. This is AOAC and MicroVal approved.


 Benefits:

  • Time to result for various matrices in 48 hours
  • Appropriate sample area to avoid spreading of the molds and ensure valid readout
  • Save space in your fridge and incubator
  • Go green and gain: reduce your environmental impact
  • Improve your inventory management with shelf life of up to 15 months.
  • Comply with regulations

Film Method - Compact Dry YM

R-Biopharm Indonesia supplies the Compact Dry YM. The ready-to-use plates consist of a special 50 mm diameter petri dish containing a detection specific nutrient pad. During growth on the nutrient medium of Compact Dry YM colonies of yeasts and molds show different color reactions and therefore they are easily to differentiate. The chromogenic substrate X-Phos initiates a blue coloration of nearly all yeast colonies. Molds will form their typical three-dimensional structures in the air space between nutrient pad and plate cover. The coloration of the air structures might be different according to the special type of mold. Growth of bacterial species on Compact Dry YM is inhibited by antibiotics which are added to the medium.


 Benefits:

  • Ready-to-use
  • No change in handling
  • No additional tools & equipment
  • Approved by AOAC, NordVal and MicroVal
  • Reliable results, easy to read and count
  • Suitable for food samples, liquids & surface swabs
  • Easy to store at RT

Enzyme Subtrade Method - Simplate Yeast and Mold

Biocontrol Indonesia utilizes the proprietary binary detection technology in Simplate to detect yeast and molds. Time to results can be in as little as 56 hours to 72 hours. Reading is easy and spreading by fast growers is overcome by using microwells to grow the yeast and molds. This gives accurate enumeration for your evaluation of the quality of your food products. All reagents are premeasured and are available in unit dose for low volume testing.


 Benefit:

  • Approved by AOAC and MicroVal
  • No media preparation
  • Faster time to result
  • Easy to interpret
  • No more serial dilution and duplicate plating
  • No spreading interference

For more question about the product please contact our expertise

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Frequently Asked Questions

  • Is it possible to have a rapid method to differentiate yeast from molds?

    Biocontrol simplate cannot differentiate between yeast and molds. This is because there is so many species. Differentiation is done by means of morphology. However the enumeration of these is difficult as some grow faster than others.

  • Why is it that everyone is testing for yeast and molds?

    This is because yeast and molds are everywhere and in the air. They contaminate every product and does not differentiate the substrate. Moldy products can easily be seen and gives a bad reputation for your products. Moreover they cause a stale taste and produces mycotoxin.

  • Why is that Simplate can detect yeast and molds faster than culture media?

    This is because Simplate detects the presence of yeast and molds by way of enzyme detection. Before they grow to colonies that is detectable by the eye, they produce enzymes for respiration growth.