ENTEROBACTERIACEAE

Enterobacteriaceae

Enterobacteriaceae is the family of gram negative, facultative anaerobic rod-shaped bacteria that is the cause of the majority of food borne disease. This large family includes many harmless bacteria that form the flora in the body and environment but also includes pathogens like Salmonella, E. coli, Yersinia pestis, Kleibsiella, Shigella, proteus, Enterobacter, Serratia and Citrobacter. The type genus is Escherichia.

Occurrence of Enterobacteriaceae

This family of bacteria is found everywhere in soil, animals, plants from insects to humans. However, many species populate the gastro intestinal tract and are as such of considerable importance to the food industry. Most species are mobile and adhere to their hosts and produce entero toxins.

Conventional Method - Biolife Italiana

Identification of the different genera of enterobacteriaceae is tedious and involves a series of tests like phenol red test, tryptone broth, phenylalanine agar, the Voges Proskauer test, nutrient gelatine test. These tests from biolife are available from Arasains Indonesia.

Film Method - Compact Dry ETB

R-Biopharm Indonesia supplies the Compact Dry ETB.  The ready-to-use plates consist of a special 50 mm diameter petri dish containing a detection specific nutrient pad. Enterobacteriaceae can’t build oxidase and can be distinguished from similar genera by this criterion.


 Benefits:

  • Ready-to-use
  • No change in handling
  • No additional tools & equipment
  • Approved by AOAC, NordVal and MicroVal
  • Reliable results, easy to read and count
  • Suitable for food samples, liquids & surface swabs
  • Easy to store at RT

Enzyme Subtrade Method - Simplate® Enterobacteriaceae

Merck Biocontrol Indonesia supplies a rapid single step method for the detection of this family of bacteria. Many European food companies uses this test as an indicator of food quality and contamination. This technology Simplate uses the proprietary binary detection technology to provide quantitative results in 24 hours. The chromogenic agar and microwells provide easy to read results.


 Benefit:

  • Approved by AOAC and MicroVal
  • No media preparation
  • Faster time to result
  • Easy to interpret
  • No more serial dilution and duplicate plating
  • No spreading interference

For more question about the product please contact our expertise

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Frequently Asked Questions

  • Conventional media test require several test but Simplate is a single test and can enumerate to 103. What is the main advantage of using Simplate over conventional culture media?

    Conventional media test require several test but Simplate is a single test and can enumerate to 103.

  • Is it not better to test for E. coli and coliforms than to test for Enterobacteriaceae?

    Enterobacteriaceae is a wide net and this gives you better safety for indicator organism. Moreover it is necessary test for European countries.

  • Is counting necessary for Enterobacteriaceae?

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